Figure 5 | Scientific Reports

Figure 5

From: Cell surface expression of GRP78 and CXCR4 is associated with childhood high-risk acute lymphoblastic leukemia at diagnostics

Figure 5

Patient-derived sGRP78+ leukemic cells disseminate in a xenotransplantation model. (A) Schematic representation of the methodology used. (B–D) sGRP78+ B-ALL human bone marrow cells were sorted from high-risk B-LLA patients and injected into a BALB/c nude immunodeficient mice. Cells from murine bone marrow (BM) and lymph node (LN) were recovered 3 weeks later and were stained with anti-human CD45 (hCD45), anti-CD34 and anti-CXCR4, representative images are depicted. (E–H) Percentages and absolute numbers of sGRP78+, CD34+ and CXCR4+ cells selected from hCD45 fraction, both in BM (E,F) and LN (G,H) are depicted. AF autofluorescence.

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