Figure 1 | Scientific Reports

Figure 1

From: Antiproliferative effects of AAV-delivered CRISPR/Cas9-based degradation of the HPV18-E6 gene in HeLa cells

Figure 1

Editing tool preparation. (A) The position of two sgRNAs and the primers used in this study. sgRNA-1 for minus strand, and sgRNA-2 for plus strand of the gene. (B) To optimized cell transduction, RFP expression was visualized directly using fluorescence microscopy (Scale bars = 50 μM). (A) HeLa cells infected with AAV-E6 sgRNAs/Cas9 virions and (B) Bright field. (C) HEK293t cells co-transfected with triple vectors PX601-mCherry, pHelper, and pAAV-RC for production of AAV-E6-CRISPR/Cas9 particles and (D) Bright field.

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