Figure 3

The effect of the gene editing on cell apoptosis (A) and proliferation (B,C). (A) Cells were double-stained with propidium iodide and annexin V-FITC, then the rate of apoptosis was monitored by flow cytometry after 5 days after infection of HeLa cells. Flow cytometry finding indicated that AAV-E6-CRISPR/Cas9 infection significantly induced apoptosis in HeLa cells compared to the control and mock cell groups. (B) MTT assay showed that disruption of the E6 gene using AAV-E6-CRISPR/Cas9 could significantly inhibit cell proliferation in HeLa cells. (C) Cell cycle profiling showed that AAV-E6-CRISPR/Cas9-mediated inactivation of the E6 gene resulted in cell cycle arrest in sub G1. The represented data are as mean ± SD independent experiments. Data were analyzed using a one-way ANOVA test. p < 0.05 was used to determine statistical significance (**p-value < 0.01 ***p-value < 0.001 ****p-value < 0.0001).