Figure 5 | Scientific Reports

Figure 5

From: Structure of Escherichia coli O157:H7 bacteriophage CBA120 tailspike protein 4 baseplate anchor and tailspike assembly domains (TSP4-N)

Figure 5

Oligomeric states of TSP4-N proteins. (A) The c(s) distribution profile of 3.6 μM oligomeric TSP4-N335 showing a single 7.7 S hexamer (dimer of trimers) peak. The insert shows the SE profile of 6.3 μM TSP4-N335 oligomer with a best fit RMSD of 0.008 absorbance units (AU), collected at 3, 6, 9 and 12 krpm rotor speeds. All SE profiles were globally analyzed using a single species of interaction system with mass conservation. The best fits are shown as black solid lines through the experimental data. The combined residuals in AU from the same cell at different rotor speeds are shown below the plot. (B) The c(s) distribution profiles of 4 μM TSP4-N490. (C) 6.8 μM TSP4-N253. (D) 8.8 μM TSP4-N181.

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