Table 3 SV results for phage CBA120 TSPs.

From: Structure of Escherichia coli O157:H7 bacteriophage CBA120 tailspike protein 4 baseplate anchor and tailspike assembly domains (TSP4-N)

Protein

Oligomeric form

MWcalc (kDa)a

S20,w (S)b

MWaap (kDa)c

f/f0b

TSP1

Trimer

248

9.6

276

1.55

TSP114-166

Trimer

50

3.6

53

1.43

TSP2

Trimer

297

9.9

279

1.56

TSP3

Trimer

206

8.4

195

1.43

Hexamer

412

12.9

357

 

TSP3156-627

Trimer

180

7.7

140

1.25

Hexamer

360

12.9

300

 

Nanomer

540

17.7

490

 

TSP4-N335

Monomer

36

2.7

39

1.46d

Trimer

109

5.2

86

1.33d

Hexamer

218

7.7

199

1.67d

TSP4-N490

Monomer

53

3.1

39

1.58

Hexamer

316

8.8

264

1.69

TSP4-N181

Hexamer

120

6.1

109

1.45

TSP4-N253

Hexamer

167

6.9

125

1.36

TSP4185-1036

Trimer

273

9.8

201

1.27

  1. aMolar mass calculated from the amino acid composition of the proteins.
  2. bS20,w and best fitted frictional ratio (f/f0) values from the SEDFIT analyses using the continuous c(s) distribution model.
  3. cExperimental molar mass distribution c(M) values from the SEDFIT analyses using the continuous c(s) distribution model. Note that the c(M) values are correlated to the f/fo values, which are subjected to the number and shapes of species present in the sample.
  4. dTSP4-N335 monomers and hexamers are purified as single species. Trimeric TSP4-N335 exists in the presence of monomeric and hexameric species.
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