Table 4 SV results for CBA120 TSP complexes.

From: Structure of Escherichia coli O157:H7 bacteriophage CBA120 tailspike protein 4 baseplate anchor and tailspike assembly domains (TSP4-N)

Protein mixture

Stoichiometry

MWcalc (kDa)a

S20,w (S)b

MWapp (kDa)

TSP1:TSP4-N335

3:6

466

11.4

431

6:6

714

14.3

615

TSP1:TSP4-N490

3:6

564

12.4

484

6:6

812

15.7

686

TSP114-166:TSP4-N335

3:6

268

8.4

139

6:6

318

11.3

220

TSP2:TSP4-N335

3:6

515

12.9

392

6:6

802

15.0

527

TSP2:TSP4-N490

3:6

613

14.3

453

6:6

910

16.7

571

TSP2:TSP4-N253

3:6

464

12.1

340

6:6

761

13.6

407

TSP2:TSP4-N181

3:6

417

12.0

292

6:6

714

13.8

413

TSP1:TSP2:TSP4-N335

6:3:6

1011

17.4

708

6:6:6

1308

19.0

870

TSP2:TSP3:TSP4-N335

3:3:6

725

15.6

603

6:6:6

1224

18.8

831

TSP1:TSP2:TSP3:TSP4-N335

6:6:3:6

1514

19.7

634

6:6:6:6

1720

23.8

847

  1. aMolar mass calculated from the amino acid composition of the proteins.
  2. bS20,w values from the SEDFIT analyses using the continuous c(s) distribution model.
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