Figure 1 | Scientific Reports

Figure 1

From: Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage

Figure 1

Light scattering calibration. (A) Histogram of NIST-traceable polystyrene beads (152, 203, 303, 401, 510, and 600 nm) are shown using side scattering (SSC-H) on a bioexponential scale using FlowJo. Each bead size relative to SSC-H is identified to obtain median side scattering in arbitrary units (a.u.) for light scattering calibration. (B) Regression plot of acquired light scattering power in arbitrary units compared to the predicted scattering cross-section in nm2 is calculated using FCMpass software. (C) Scatter-diameter curve showing light scatter intensity relationships with EV diameter established in FCMpass software. The acquired NIST-traceable polystyrene bead scattering intensity are overlaid with the predicted scattering data for NIST-traceable polystyrene beads with refractive index of 1.5900. The scatter-diameter relationship given high, average, and low effective EV refractive indices are shown, which can be used to estimate EV diameter from corresponding scattering intensity in arbitrary units.

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