Figure 6 | Scientific Reports

Figure 6

From: Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage

Figure 6

Effect of freeze thaw and post-thaw processing on cf-mRNAs using qRT-PCR. (A) Hierarchical clustering analysis of relative levels (in ΔCt) of 16 custom selected genes using RT-qPCR. Ct difference (ΔCt) between S1 and individual processing conditions are indicated from lowest (blue) to highest (red) using R. Non-tissue specific genes that are fully or partially removed, and tissue-specific genes which are retained in S1FRS2 with respect to S1 are shown. (B) Box plot of the median expression levels (in Cts) for representative non-tissue specific genes which are fully removed (i.e. HBG1 and SMC4) or partially removed (i.e. PF4, and B2M), and tissue-specific genes (i.e. APOE, ALB) which are retained in S1FRS2 with respect to S1 are shown using R. Higher raw Ct indicates lower levels of cfRNA transcripts.

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