Figure 2 | Scientific Reports

Figure 2

From: Development and validation of a high throughput SARS-CoV-2 whole genome sequencing workflow in a clinical laboratory

Figure 2

Comparison of amplicon coverage balance. A bar plot depicting the average sequencing depth of the amplicons (A) using equimolar primer pools, low performing amplicons were observed; (C) optimized modified primer pools, coverage of low performing amplicons was improved; (B, D) ARTIC v3 standard primers with even coverage. A same clinical sample set was used for plot (A) and (B) (Ct 24, n = 11); and (C) and (D) (Ct 24, n = 5). All samples were normalized to 200,000 mapped reads for comparison.

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