Figure 4
From: PPE51 mediates uptake of trehalose across the mycomembrane of Mycobacterium tuberculosis

Growth of the Mtb Δppe51 deletion mutant in minimal medium with glucose or trehalose as the sole carbon source. Cells of Mtb H37Rv wild-type (WT) (red bars), the Δppe51 mutant (green bars) and the complemented mutant Δppe51 pMV361::ppe51 (blue bars) were cultivated in minimal medium containing increasing concentrations (0–5 mM) of trehalose (A) or glucose (B), respectively. Growth was determined using the resazurin microplate assay and normalized to cells of Mtb H37Rv wild-type grown in Middlebrook 7H9 complete medium (= 100% growth) or medium only control (= 0% growth). Values are means of triplicates ± SD. Growth defects of the Δppe51 mutant observed at 5 mM concentration of both sugars compared to wild-type or the complemented mutant was statistically significant (p < 0.005 as determined using unpaired t test).