Figure 5 | Scientific Reports

Figure 5

From: PPE51 mediates uptake of trehalose across the mycomembrane of Mycobacterium tuberculosis

Figure 5

Growth of the Mtb Δppe51 deletion mutant in 7H9 limited medium with glycerol or trehalose as the sole carbon source. Cells of Mtb H37Rv wild-type (WT), the Δppe51 mutant and the complemented mutant Δppe51 pMV361::ppe51 were cultivated in 7H9 limited medium containing 0.5% (v/v) glycerol (blue bars), 10 mM trehalose (red bars) or no additional carbon source (brown bars). Strains grown in 7H9 complete medium served as positive controls (green bars). Growth was determined using the resazurin microplate assay and normalized to cells of Mtb H37Rv wild-type grown in 7H9 complete medium (= 100% growth) or medium only control (= 0% growth). Values are means of two independent experiments each performed in triplicates ± SD. Growth differences between the indicated groups were statistically significant (***p < 0.005 as determined using unpaired t test).

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