Figure 6
From: PPE51 mediates uptake of trehalose across the mycomembrane of Mycobacterium tuberculosis

Metabolic labelling of Mtb strains employing click chemistry proves PPE51-dependent uptake of 6-TreAz. Mtb strains were incubated with 100 µM 6-TreAz for 3 days and subsequently conjugated with AF488 DIBO alkyne. Mean fluorescence intensity (MFI) was measured for 50,000 cells from each sample in each experiment. Data shown is the mean of three independent experiments ± SD. Differences in MFI between the indicated groups were statistically significant (***p < 0.005 as determined using unpaired t test). The high background in the controls that were not incubated with 6-TreAz is presumably due to non-specific association of the secondary DIBO-fluorophore.