Table 1 Results from embryo transfers (ETs) of zygotes injected 6 h post insemination with in vitro transcribed (IVT) or synthetic gRNAs and Cas9 protein.
From: A deletion at the polled PC locus alone is not sufficient to cause a polled phenotype in cattle
ET | gRNA type | Blastocysts transferred | Recipients | 35 days of gestation | 80 days of gestation | Fetuses harvested (%) | ||
|---|---|---|---|---|---|---|---|---|
Pregnant (%) | Fetuses detected (%) | Pregnant (%) | Fetuses detected (%) | |||||
1 | IVT | 18 | 6 | 2 (33) | 4 (22) | 2 (33) | 4 (22) | 3 (17) |
2 | IVT | 24 | 8 | 5 (63) | 8–9 (33–38) | 1 (13) | 1 (4) | 1 (4) |
Total | IVT | 42 | 14 | 7 (50) | 12–13 (29–31) | 3 (21) | 5 (12) | 4 (10) |
3 | Synthetic | 12 | 4 | 0 (0) | 0 (0) | – | – | – |
4 | Synthetic | 24 | 10 | 1 (10) | 3 (8) | 1 (7) | 3 (13) | 3 (13) |
Total | Synthetic | 36 | 14 | 1 (7) | 3 (8) | 1 (7) | 3 (8) | 3 (8) |
Overall total | IVT & Synthetic | 78 | 28 | 8 (29) | 15–16 (19–21) | 4 (14) | 8 (10) | 7 (9) |
