Figure 5
ILF2 enhances A3B cytidine deaminase activity. (a) Immunoblot analysis of interactor overexpressing HEK293T cell lysate (upper panel) and representative TBE urea PAGE analysis of A3B cytidine deaminase activity (lower panel). LaminB1 is an endogenous control. (b) Quantification of the cytidine deaminase activity data in five biologically independent experiments. Deaminated/total (deaminated and aminated) percentage of product was calculated and normalized by the control sample overexpressing A3B without interactors. (c) Immunoblot analysis of interactor knocked down HEK293T cell lysate and representative TBE urea PAGE analysis of A3B cytidine deaminase activity. (d) Quantification of the cytidine deaminase activity data in five biologically independent experiments. Deaminated/total (deaminated and aminated) percentage of product was calculated and normalized by the sample overexpressing A3B with control siRNA. Values are means standard deviations (error bar). Asterisks (*) show statistically significant difference p < 0.05). EV Empty vector, NC Negative control (scramble siRNA). Original blots and gels are presented in Supplementary Figure S15.
