Figure 2

Dephosphorylated ASCL1 promotes neuronal differentiation. (A–C) Immunostaining for the neuronal marker TUBB3 (red) and quantification of the % of TUBB3⁺ cells over the total DAPI⁺ cells cultured for 7 (A,B) or 16 days (C,D) without growth factors and in the presence of dox to induce ASCL1 expression. Scale bars: 100 μm. Data: mean ± SEM n = 3 independent experiments; one-way ANOVA followed by the Bonferroni post-hoc test; **p < 0.01; ***p < 0.001. (E,F) Relative mRNA expression of different neuronal (E) and oligodendrocyte (F) markers at 7 days. Data: mean ± SEM, normalized to TBP. n = 3 independent experiments; one-way ANOVA followed by the Bonferroni post-hoc test; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.