Figure 3
From: ASCL1 phosphorylation and ID2 upregulation are roadblocks to glioblastoma stem cell differentiation
Dephosphorylated ASCL1 promotes stable mitotic exit and differentiation. (A) Representative images of G144 cells after removing ASCL1 induction and re-addition of growth factors. Scale bars: 300 μm. (B) Quantification of cell confluence. Data: mean ± SEM n = 2 independent experiments for Control + GFs and n = 3 independent experiments for the other conditions; one-way ANOVA followed by the Bonferroni post-hoc test; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. All data points from day 3 onwards are statistically different between control and WT ASCL1 and between control and 5S-A ASCL1 (p < 0.05; not shown). (C) Immunostaining showing persistent expression of the neuronal marker TUBB3 (red) at 25 days of culture. Scale bars: 50 μm.
