Figure 7
(a)–(c) Representative images of gonadotropin-releasing hormone 3 (GnRH3) neurons (red) in the following four groups of brain slices: control, inhibitor-treated, 11-ketotestosterone (11-KT)-treated (10 nM), and 11-KT- (10 nM) and inhibitor-treated groups. Cell nuclei (blue) were stained with Hoechst 33342. (d)–(f) The numbers of GnRH3 neurons in the four groups. Control and inhibitor-treated slices originated from the same animals, while 11-KT-treated slices and 11-KT and inhibitor-treated slices originated from the same animals. Inhibitors used in (a)–(c) and (d)–(f) were as follows: (a, d) phosphoinositide 3-kinase (PI3K) inhibitor (LY294002), (b, e) AKT inhibitor (GDC-0068), (c, f) mechanistic target of rapamycin (mTOR) inhibitor (rapamycin). Results from two-way analysis of variance (ANOVA) are shown in the top. Numerals in bars indicate the numbers of animals used for preparing slices. #, a significant difference at p < 0.05 (two-way ANOVA). * and **, a significant difference at p < 0.025 and p < 0.005, respectively [paired t-test, Bonferroni Correction (α new = α original / n = 0.05/2 and 0.01/2]. Data are expressed as the mean ± standard error of the mean.
