Table 4 Sequences of the primers used for cloning of SeSOS1 and quantitative real-time PCR.
Gene | Primer | Sequence 5′–3′ | Amplicon size (bp) | References |
|---|---|---|---|---|
SeSOS1 degenerate primers | deg1_F | ACGCBGTBATCTTCKTCGGART | ca. 1800 | This study |
deg1_R | AGAAADGCAGCACABATRARC | |||
deg2_F | GNGGTACTMGVGTNCCCTAYACTG | ca. 1300 | ||
deg2_R | CCTAGYTCYTCRTCDTCHCGAAGATC | |||
deg3_F | TCAATGGAARTTCAYCARATAAAG | ca. 1500 | ||
deg3_R | GCWGCTTGMACACCATTYARVARSCG | |||
SeNHX1 | Forward | GGAGAATCGTTGGATGAATGAGTC | 182 | |
Reverse | GCTTCTTTTTTACCTGGAACCCTG | |||
SeSOS1 | Forward | GCGGTTGGAGTTTTGTTCCC | 110 | This study |
Reverse | AGGGATAATGCCACAGCTCC | |||
SeCAC | Forward | CGTGCCTTCTGATGCGACTA | 166 | |
Reverse | TGCCTCTTCACTTGTGATGCT |
