Figure 5

Representative images of histological analysis of DNA damage in excised human skin. Paraffin sections are stained for 6-4PP (left column) and CPD damage (right column). Positive cells are stained in dark red. Non-irradiated human skin (negative control) shows no positive cells. Human skin irradiated with 3 mJ/cm2 UVB (0.1 MED) showed > 80% CPD damage throughout the whole epidermis. The CPD damage after 40 mJ/cm2 irradiation at 222 nm and 233 nm only occurred on the uppermost layer of the epidermis. Scale bar: 100 µm.