Figure 4

Fostamatinib treatment did not affect B cell or plasma cell populations. Flow cytometry was used to measure mature B lymphocytes populations in fostamatinib treated and vehicle treated sensitized LEW rats. Each graph shows number of cells per gram of spleen. Mature B lymphocyte numbers remained similar between treatment groups. (A) Memory cells were defined as CD45R⁺CD27⁺, (C) switched cells as CD45R⁺CD27⁺IgD⁻, (D) non-switched cells CD45R⁻CD27⁺ IgD⁺, (F) plasma cells as IgD⁻CD45R⁻IgM⁻CD138⁺. (n = 6 rats/group). Graphs show cell numbers per gram of spleen (n = 6 rats/group). Representative flow cytometry data of (B) memory B lymphocytes (E) switched and non-switched B lymphocytes (G) plasma cells. Number shown represents percentage of cells in gate. Blue filled circle—vehicle, red filled triangle—fostamatinib.