Figure 1
From: Effect of substrate stiffness on friction in collective cell migration
Effect of substrate stiffness on cell–substrate and cell–cell adhesions. (a) Confocal images of HaCaTs stained for vinculin (green) and actin (red) on substrates having moduli of 6, 18, and 27 kPa. (b) Effect of substrate stiffness on normalized vinculin area. (c) Confocal images of HaCaTs stained for E-cadherin (green) and actin (red) for varied substrate stiffness. (d) Relative E-cadherin intensity (difference between junctional and cytoplasmic intensities divided by the sum) for varied substrate stiffness. No statistical effect of substrate stiffness on relative intensity was present. For panels (b,d), each data point represents an average over a statistically independent field of view. Horizontal lines indicate means.
