Figure 3
From: Effect of substrate stiffness on friction in collective cell migration
Effect of time in culture on cell–substrate and cell–cell adhesions. (a) Confocal images of HaCaTs stained for vinculin (green) and actin (red) after 12, 24, or 48 h in culture on substrates of Young’s moduli 6 kPa. (b) Effect of time in culture on normalized vinculin area. (c) Confocal images of HaCaTs stained for E-cadherin (green) and actin (red) for varied times in culture. (d) Relative E-cadherin intensity (difference between junctional and cytoplasmic intensity divided by the sum) for varied time in culture. For panels (b,d), each data point represents an average over a statistically independent field of view. Horizontal lines indicate means.
