Figure 4
Changes in the reserved respiratory capacity (a), coupling efficiency (b), basal respiration (c), maximal respiratory capacity (d) and ATP synthesis (e) of HCT116 cells associated with miRNA mimic transfections in combination with metformin treatment. CRC cells were reverse transfected with selected miRNA mimics and treated with 2.5 mM metformin treatment or control medium. Oxygen consumption rate (OCR) of the cells was determined over a 30-min period using Seahorse XFe-96 instrument. The OCR values were normalized to viability of the corresponding cells acquired by crystal violet assay. Results are expressed as mean ± SD of 3 culture replicates and the statistical significance is indicated with asterisks (ns P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001).
