Figure 6
Cell cycle distribution of HCT116 cells after transfection with sensitizing miRNAs and treatment with metformin or control medium for 96 h (a) and the miRNA-based sub-network of putative target genes associated with cell cycle (b). (a) Histograms showing the percentage of HCT116 cells transfected with miR-18b-5p, miR-145-3p, miR-376b-5p, miR-676-3p and miR-718 mimics and treated with 2.5 mM metformin for 72 h in G0/G1, S, and G2/M phases, compared with cells in control medium. A “–” miRNA mimic status indicates transfection with NC negative control mimics. Results are expressed as mean ± SD of 3 replicates and the statistical significance is indicated with asterisks (ns P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001). (b) This is an organic layout of the integrated network associated putative genes organised in cell cycle associated ontologies acquired by Targetscan predition algorithm and NetworkAnalyst-Panther/Slim GO analysis. The miRNA-gene interactions are shown as red lines while gene–gene interactions are green. The blue and red nodes represent predicted taget genes and miRNAs, respectively.
