Figure 3
(a) Real-time colorimetric curves corresponding to mut:wt starting template ratios spanning more than four orders of magnitude. (b) Calibration curve produced from real-time data of triplicate colorimetric LAMP measurements with error bars representing standard deviation; y = − 1.165ln(x) + 23.72 (R2 = 0.99). (c) Real-time data corresponding to tissue samples of various mut:wt ratios. The 1% was not consistently produced (yellow vs green line). (d) Comparison of real-time data for 10, 20 and 50% mut:wt tissue samples measured directly in the qcLAMPdevice without DNA purification. (e) Real time colorimetric LAMP results of 12 patient samples analyzed with the qcLAMP 3D device (blind tests). (f) Comparison of ddPCR, Sanger and qcLAMP test results of 12 clinical samples. The ddPCR method provided additional information regarding the concentration of mutant and wild type copies per analyzed sample.
