Figure 1
From: MqsR toxin as a biotechnological tool for plant pathogen bacterial control
In vitro analysis of purified MqsR in X. citri. (a) Immunoblot detection of MqsR associated with X. citri cells. Four treatments were examined: WC – washed X. citri control; C—non-washed X. citri control; WT—washed X. citri treated with MqsR; and T—non-washed X. citri treated with MqsR. PT is purified MqsR (positive control). The values 0.5 and 1.0 correspond to the initial OD used, and 14 kDa is the expected size of MqsR with a His(6)-tag. Equivalent proteins loading was verified with Coomassie Brilliant Blue (CBB). In this case, two gels were run in parallel, where one was stained with Coomassie blue to show the concentration of proteins (Supplementary Fig. S1) and the other was used for western blot. (b) Growth curve of X. citri in the presence of MqsR. Five treatments were evaluated, X. citri treated with 25, 50, 100 and 200 µg/mL of MqsR and the non-treated control. After 10 h, the concentrations of 50, 100 and 200 µg mL-1 showed significantly lower growth than the non-treated control (P < 0.05). Full-length gels and blots are included in Supplemental information. (c) Growth curve of X. citri and X. citri overexpressing MqsA (X. citri :: MqsA) in the presence of 50 and 100 µg/mL of MqsR and the non-treated control. After 10 h, only the concentration of 100 µg mL-1 showed significantly lower growth in X. citri :: MqsA than the non-treated control (P < 0.05).
