Figure 3
From: MqsR toxin as a biotechnological tool for plant pathogen bacterial control
Citrus canker symptoms and MqsR detection in X. citri_GFP. (a) Citrus canker symptoms were evaluated at 14 DAI in wild-type and transgenic lines (top). The red squares correspond to the same area visualized by fluorescence microscopy (bottom). Green labelling indicates X. citri_GFP in the leaves. Pi_WT, wild-type Pineapple sweet orange; Pi_mqsR_1, 2, 3 and 4, transgenic Pineapple sweet orange lines; C_WT, wild-type Carrizo; and C_mqsR, transgenic Carrizo. (b) Detection of MqsR in X. citri_GFP cells by immunoblotting. The bacteria used in this experiment were isolated from the following plant lines: WT, wild-type; Transgenic (Pi_mqsR_1) representative line; leaf infiltrated with PBS (control); and, leaf infiltrated with X. citri_GFP (X. citri). MqsR corresponds to purified protein (positive control) for which the expected size is 14 kDa with a His(6)-tag. (c) Cells correspond to total proteins from X. citri_GFP (positive control). The expected size of GFP is 26.9 kDa. Full-length gels and blots are included in Supplemental information.
