Figure 5

Diversity of the farming animals’ gut microbiota. PCoA of the unweighted UniFrac distances showing (A) all samples, a significant separation among animal groups was observed (permutation test with pseudo-F ratios (Adonis); p < 0.001). Black arrows were obtained by fitting the relative abundance of bacterial families for each sample within the ordination space (function envfit of the vegan R package). (B) Boxplots showing alpha diversity values according to the different metrics: Faith's phylogenetic diversity (PD_whole_tree), Chao1 index and OTU species count (observed_species). All metrics showed a significantly higher gut microbiota diversity for Donkey, Pig and Horse (Wilcoxon rank-sum test; p < 0.05).