Figure 2
From: Simple methods for quantifying super-resolved cortical actin
Simulation of meshworks for analysis testing. (a) Initial output of 1px wide lines forming a simulated mesh. (b) Image (a) after dilation of lines to 7px and binning of the image, to match the pixel size of the cameras on our SIM system. (c) Convolution with Gaussian blur, as calculated from an estimated PSF based on our optics. (d) Application of Poisson and Gaussian noise to mimic noise from the camera. (e) Smoothing of (d). (f) A TIRF image for comparison of the same scale as the simulated data (scale bar = 1 μm). (g) Ground truth simulated meshwork (scale bar 1.5 µm), shown in (h) after convolution and application of our meshwork analysis. (i) Comparison of mean area ± standard deviation of identified corrals from over 30 repeats of network simulation against the ground truth.
