Figure 4
From: Simple methods for quantifying super-resolved cortical actin

Analysis is applicable to live cell data. (a) Example SRRF frame from a 10 s live stack (1 fps) from a LifeAct mEGFP expressing A549 cell. (b) Projected overlay of all corrals identified over the 10 frame stack (see Supplementary Video 1). (c) Plot of the area of the corral marked with * in a) over time. (d) Frame by frame images of the corral marked in (a) and plotted in (c), showing the raw and processed image.