Figure 5
From: Simple methods for quantifying super-resolved cortical actin
Example of analysis of expanded actin. (a) Example of 3D-SIM imaged expanded actin, with a scale bar of 10 μm (uncorrected for expansion factor). (b) Randomly generated line across the image for analysis, where intensity along this line is plotted in (d). (c) Example of the distribution of randomly generated analysed lines when repeated 100 times. (d) Normalised intensity line plot of fluorescence intensity (a.u.) vs distance along line for the line shown in (b), with peaks identified and height and width denoted as in the key. (e) Enlarged region of box in (d), showing identification of all distinguishable peaks above a manually set threshold.
