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Figure 2

From: Inhibition of HIF-1α-AQP4 axis ameliorates brain edema and neurological functional deficits in a rat controlled cortical injury (CCI) model

Figure 2

Inhibition of HIF-1α reduced brain water content and improve blood–brain barrier function. TBI rats were intravenously injected via the tail vein with 0.25 ml isotonic saline containing 2-ME2 (2.5 mg/kg of body weight) within 30–60 min after TBI, the corresponding controls were injected with isotonic saline. On the 1st day after injury, brain water content (B) was determined and protein levels of HIF-1α (A), AQP4 (C), MMP-9 (D), VEGF (E), tight junction protein levels of occluding (F) and claudin-5 (G) were detected by western blot. β-actin was used as loading controls for the total proteins. Data represent mean ± SEM (n = 6 per group). The comparisons among groups were analyzed by one-way ANOVA and the LSD method. Significant differences are shown by asterisks (*P < 0.05). ns non-significant.

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