Figure 4 | Scientific Reports

Figure 4

From: Inhibition of HIF-1α-AQP4 axis ameliorates brain edema and neurological functional deficits in a rat controlled cortical injury (CCI) model

Figure 4

Effects of AQP4 and HIF-1α inhibition on neuron loss and the expression levels of neuron structure-related proteins in hippocampus. TBI rats were intravenously injected via the tail vein with 0.25 ml isotonic saline containing AQP4 antibodies (1 μg/kg of body weight) or 2-ME2 (2.5 mg/kg of body weight) within 30–60 min after impact, the corresponding controls were injected with IgG or isotonic saline, respectively. On the 21st day post-TBI, (A) the hippocampal CA1 NeuN-positive neuron numbers of the injured region in each group were calculated (scale bar 100 μm). The hippocampal protein levels of MAP2 (B) and SYN (C) were detected by western blot. (D) The protein expression of pTau (ser404) and Tau-5 was detected in each group. β-actin was used as loading controls for the total proteins. Data represent mean ± SEM, n = 6 per group. The comparisons among groups were analyzed by one-way ANOVA with the LSD method. * P < 0.05, between the two groups. ns non-significant.

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