Figure 4

Characteristic and transcriptional expression of IbHCT/HQTs. (A) Phylogenetic analysis of IbHCT/HQTs and other BAHD proteins. The phylogenetic tree was established with neighbor-joining method by using program MEGA (Version 9.0, https://www.megasoftware.netExternal Link) with 1000 bootstrap replications. (B) Sequence alignment of IbHCT/HQTs. The amino acids in the box represent the conserved motifs HXXXD and DFGWG. The amino-acid sequences of BAHD proteins used in phylogenetic analysis and sequence alignment are listed in (Supplementary Table S9. Multiple alignments of amino acid sequences were performed using DNAMAN (version 8.0). (C) Relative expression levels of IbHCT/HQTs in different sweet potato tissues by qRT-PCR. The values are means ± SD of three independent biological replicates.