Figure 2
From: Panel of human cell lines with human/mouse artificial chromosomes
Representative images of FISH analyses of HEK293 cells, HT1080 cells, hiMSCs, and iPSCs that contained each HAC/MAC. (a) HEK293 cells that contained 21HAC1. Red: alpha satellite probe (p11-4) staining the centromere of Chr.13, 21, 21HAC1. (b) HEK293 cells that contained 21HAC2. Red: alpha satellite probe (p11-4) staining the centromere of Chr.13, 21 and 21HAC2; green: pCX-EGFP. (c) HEK293 cells that contained MAC2. Red: mouse cot-1 staining MAC2. (d) HEK293 cells that contained MAC4. Red: mouse cot-1 staining MAC4. (e) HT1080 cells that contained 21HAC2. Red: alpha satellite probe (p11-4) staining the centromere of Chr.13, 21, 21HAC1. (f) HT1080 cells that contained MAC4. Red: mouse cot-1 staining MAC4. (g) hiMSCs that contained 21HAC2. Red: alpha satellite probe (p11-4) staining the centromere of Chr.13, 21 and 21HAC2. (h) hiPSCs (201B7) that contained MAC6. Red; mouse cot-1 staining MAC6. Inset: enlarged images of each HAC or MAC.
