Figure 10

E6 activity on P-HES1 regulated genes. The ratio of mean fluorescence intensity (MFI) of DsRed2 and mTagBFP2 were calculated for each mTagBFP2-E6 based on the triple gating strategy as described in Fig. 6 and normalized to the control mTagBFP2, which has no effect on P-HES1 controlled DsRed2 expression (ratio set to zero). The more negative or positive the value the higher the repressing or activating activity of the respective E6. Data are derived from the average of three independent biological replicates with the mean value labelled above each bar. The error bars plotted are the standard deviation of the mean from the three independent biological replicates. P value were calculated using One-Way ANOVA with Fischer’s LSD test by comparing the mean of each activation and repression sample with the control activation sample where * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.005, **** = P ≤ 0.0001, ns = P > 0.05.