Figure 3 | Scientific Reports

Figure 3

From: An enhanced triple fluorescence flow-cytometry-based assay shows differential activation of the Notch signaling pathway by human papillomavirus E6 proteins

Figure 3

Schematic triple gating strategy. Living cells are first identified by FSC/SSC gating followed by specific inclusions of cells that are transfected with the activator plasmid (EGFP + cells). Next step is to gate on EGFP/mTagBFP2 to only monitor cells that have been transfected with the activator and the modulator (EGFP + /mTagBFP2 + cells). Then, Notch-activation is assessed in this population by measuring the DsRed2 expression (EGFP + /mTagBFP2 + /DsRed2 + cells). Picture was created with BioRender.com.

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