Figure 5 | Scientific Reports

Figure 5

From: An enhanced triple fluorescence flow-cytometry-based assay shows differential activation of the Notch signaling pathway by human papillomavirus E6 proteins

Figure 5

Cross talk of fluorescent proteins. (a). Representative FACS plots showing the crosstalk level of the three fluorescence proteins after compensation. C33A cells were transfected with control plasmid 250 ng EGFP, 125 ng mTagBFP2 or 125 ng DsRed2 individually. FACS measurement was carried out 48 h post transfection. Living cells (P1) were gated for EGFP, mTagBFP2 and DsRed2 separately to monitor the crosstalk of each fluorescence proteins in respective channel showing cell populations in % cells. EGFP showed a compensable crosstalk signal in the channel of mTagBFP2 and DsRed2. Crosstalk levels were always kept below 0.5% of cell populations in each experiment. (b, c, d). The experiment was repeated three times. The mean % cells of cell populations as gated of three independent biological experiments are plotted in b, c and d with the mean value stated above each bar. The error bars plotted are indicating the standard deviation of mean value from the three independent biological replicates.

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