Figure 1

Optical and electronic setup diagram of FOCALS microscopy. In the diagram, the red-colored optical beam corresponds to the FLIM and SHG excitation source (760 ± 5 nm), the green-colored optical beam corresponds to the OCM excitation source (760 ± 80 nm), and the blue-colored optical beam corresponds to the combined OCM-FLIM (and SHG) excitation beams. ↕ and ⊙ correspond to vertical and horizontal polarization states, respectively, and the dashed lines near the beam-spillters indicate the direction of beam propagation (that are pertinent to the excitation and detection paths of FOCALS microscopy).