Figure 5 | Scientific Reports

Figure 5

From: Label-free metabolic and structural profiling of dynamic biological samples using multimodal optical microscopy with sensorless adaptive optics

Figure 5

Tissue profiling with FOCALS microscopy. (a) Contour lines of the NAD(P)H autofluorescence lifetime data for the different tissues shown in Figs. 2, 3 and 4 on the phasor plot, where the threshold was set to be g > 0.05. (b–c) Beeswarm plots of the SHG intensity (normalized to 20 a.u. and in log scale) and the polarization ratio of pixels with SHG intensity greater than the 25th percentile of each frame. (d) Radar plot to show the multidimensional distribution of the quantities from FOCALS microscopy for the spleen, tail, tumor, skin, and muscle. The SHG intensities for the hippocampus, liver, and kidney were too weak for visualization or quantification; therefore, they were neglected in (b–d).

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