Figure 2

Effect of Amphetamine on monoamine efflux in PFC tissue. (A) PFC tissue slices from WT, DAT-KO and NET-KO mice were treated with amphetamine (AMPH, 10uM), and monoamines released into the KH incubation buffer were analyzed by HPLC. For each monoamine, Amph-induced release was normalized to KCl-induced release (shown in B) for each respective genotype and then normalized to WT levels. F (2, 42) = 7.325 for genotype comparisons. ****P < 0.0001 using two-way ANOVA, compared to WT mice. n = 2 experiments with 3 mice in each experiment. (B) PFC tissue slices from WT, DAT-KO and NET-KO mice were treated with 40 mM KCl, and monoamines released into the KH incubation buffer were analyzed by HPLC. For each monoamine, KCl-induced release was normalized to WT levels. ns Not significant using two-way ANOVA, compared to WT mice. n = 3–4 experiments with 4 mice in each experiment.