Figure 3

Effect of monoamine transporter blockers on monoamine efflux in PFC tissue. PFC tissue slices from WT, DAT-KO and NET-KO mice were treated with KCl alone (40 mM) or KCl + 10 µM desipramine, GBR12909 (GBR) or fluoxetine. Monoamines released into the KH incubation buffer were analyzed by HPLC. F (3, 21) = 3.712 WT NE, F (3, 34) = 3.284 WT DA, F (3, 35) = 5.957 WT 5-HT, F (3, 32) = 10.85 DAT-KO DA, F (3, 24) = 5.003 DAT-KO NE, F (3, 20) = 8.410 DAT-KO 5-HT, F (3, 24) = 0.5012 NET-KO DA, F (3, 24) = 0.4536 NET-KO NE, F (3, 35) = 3.578 NET-KO 5-HT, for drug treatment comparisons. *P < 0.05, **P < 0.01, ****P < 0.0001 using two-way ANOVA, compared to KCl-treated controls. n = 3–4 experiments with 4 mice in each experiment.