Figure 2 | Scientific Reports

Figure 2

From: Salivary protein 7 of the brown planthopper functions as an effector for mediating tricin metabolism in rice plants

Figure 2

Relationship between NlSP7 and tricin. (A) Tricin mass concentration (MC) (ng/ml) analysis results at the three-leaf stage of seven rice varieties: Taichung Native 1 (TN1), Mudgo, IR26, ASD7, IR36, IR56, and Rathu Heenati (RH) carrying different genes resistant to brown planthoppers. (B) Tricin mass concentration of tricin in the root, stem and leaf parts of RH and TN1 rice varieties. One g each of fresh root, stem, and leaf was ground, and the content of tricin in each part was measured using liquid chromatography-mass spectrometry. Data are represented as the mean ± SD from three biological replicates. (C) q-PCR was used to determine the expression level of TN1-P and RH-P fed two different varieties of rice plants. (D) Artificial diets wih tricin and without tricin were fed to brown planthoppers, then q-PCR was used to detect the expression level of NlSP7 at 2, 4, 8, 12, and 24 h. The mean amounts of three biological replicates (mean ± SD, n = 10) are shown, ** P < 0.01. (Student’s t-test).

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