Figure 3

RNA interference (RNAi) effects on feeding of brown planthoppers (BPHs) exposed to three different treatments (control, injected with dsGFP, injected with dsNlSP7) and resistance levels of different rice varieties. (A) On Taichung Native 1 (TN1) and Rathu Heenati (RH) rice varieties, the mean honeydew amounts excreted at 24 h by a female adult BPH that received three treatments. The experiment was repeated with 20 replicates. (B) Weight gain of BPHs fed TN1 and RH rice varieties after injection with different dsRNA treatments. (C) Mean weight of BPHs fed LDS-sucrose and LDS-tricin (mean ± SD, n = 20). Letters indicate significant differences (P < 0.01, Duncan’s multiple range test). (D) Rice resistance levels of BPHs fed TN1 and RH rice varieties. The experiment was repeated three times. Data are represented as the mean ± SD (P < 0.01, Duncan’s multiple range test). (E) mortality rate of BPHs fed RH rice, repeated 10 times, respectively. (F) mortality rate of BPHs fed an artificial diets with 100 mg/l tricin, repeated 10 times. Asterisks (*) indicate significant difference (P < 0.05, Student’s t-test).