Figure 7
The effect of brown planthoppers in three groups (control, injected with dsGFP, injected with dsNlSP7) on the tricin content and tricin metabolic pathway genes of different rice varieties. (A) Taichung Native 1 (TN1) and (B) Rathu Heenati (RH) rice varieties fed on by brown planthoppers. After 3 days, the content of tricin was determined by liquid chromatography-mass spectrometry, A same letters above the bars indicate no significant differences between three treatment groups, and B different letters above the bars indicate significant differences between BPHs injected with dsNlSP7 and two control groups (control and dsGFP). Data are represented as the mean ± SD from three biological replicates (P < 0.01, Duncan’s multiple range test). (C) The q-PCR used to determine the expression level of tricin metabolic pathway genes (PAL, C4H, 4CL, CHS, CHI) in rice after being fed on by brown planthoppers. The experiment was repeated three times.
