Figure 3
From: Shared genetic loci for body fat storage and adipocyte lipolysis in humans
Effects of RNA interference-mediated knock-down of candidate genes in human human adipose-derived stem cells. Human adipose-derived stem cells were transfected with control siRNA (siNegC) or indicated siRNAs targeting specific genes (ZNF436, STX17, GGA3 and NUP85) and followed until differentiation day 6 and 9, upon which the expression of target genes, LIPE, PLIN1, PNPLA2 and ADIPOQ was monitored. Relative gene expression was normalized to the reference gene 18s. Results were analyzed using t-test and presented as fold change ± SD relative to control siRNA (NegC) of corresponding time point (n = 6, for GGA3 n = 11). ***p < 0.005, **p < 0.01, *p < 0.05.
