Figure 2
From: Evaluation of phototoxicity induced by the anticancer drug rucaparib
(a) Emission spectra (λexc = 320 nm) of rucaparib (RCP) after internalization on HaCaT cells (red), RCP in PBS solution (black) and anthracene (grey) in ethanol. The latter was used as standard. Isoabsorptive solutions for all conditions were prepared and then, fluorescence was recorded. (b) Intracellular localization of RCP in HaCaT cells by confocal microscopy. Keratinocytes, seeded on glass coverslips, were incubated with 20 µM solutions of RCP (green) and labelled with CellMask Orange Plasma membrane (red) and RedDot1 Far-Red nuclear (blue). Colours were chosen arbitrarily. Emission spectra were drawn using GraphPad Prism v5.03.
