Figure 6

Unexposed HaCaT cells alone or treated with 10 μM of chlorpromazine (CPZ, positive control), 100 μM of 5-hydroxydiclofenac (5-OH DCF, recovery positive control) or 50 μM of rucaparib (RCP). Cells were kept on dark conditions or irradiated with a 2 J/cm² UVA dose. (a) Representative fluorescence microscopy images of cells treated with RCP or 5-OH DCF. (b) Percentage of DNA damage by visual scoring of untreated cells (HaCaT) or treated with CPZ, 5-OH DCF or RCP. Data represent mean ± SD of four independent experiments upon dark (■), UVA light (□) or UVA light + 6 h of time recovery conditions. Asterisks indicate significant differences by Student’s t test (***p < 0.001, **p < 0.001, *p < 0.05, ns non-significant). Visual scoring of DNA damage was established using ImageJ v1.52p. The percentage of DNA damage was represented using GraphPad Prism v5.03.