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Figure 1

From: Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism

Figure 1

Analyses on morphology, fresh weight and total fatty acid content on embryos or seeds at mid-developmental stage. (A) Photos of embryos at middle stage, 38 days after pollination (DAP) in Hong Kong. Scale bar = 200 μm. RNA-seq normalized counts for the respective transgenes are shown to the right of the images. Transgene expression was not detected in WT samples, except for one read for AtGPAT9 which mapped to an endogenous B. napus GPAT in the WT sample. (B) Fresh seed weight at 38 DAP. Values are means ± S.E. (n = 17–45). (C) Total fatty acid (FA) content on the basis of seed number at 38 DAP. *Indicates significant differences (P < 0.05) from wild type (DH12075) by Student’s t test. (D) Relative abundance (mol%) of PC molecular species and (E) TAG molecular species determined by MALDI-MS in fully mature whole embryos.

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