Table 1 Incubation conditions during the BAY-747 and runcaciguat ex vivo cLTP experiments.

From: The sGC stimulator BAY-747 and activator runcaciguat can enhance memory in vivo via differential hippocampal plasticity mechanisms

Experimental condition

Treatment during 10 min BAY-747 incubation

Treatment during cLTP protocol

Vehicle

0.1% DMSO

0.3% DMSO

BAY-747 only (without WS)

100 nM BAY-747

0.3% DMSO

BAY-747 + WS

100 nM BAY-747

50 µM forskolin + 0.1 µM rolipram

Weak stimulation (WS) only

0.1% DMSO

50 µM forskolin + 0.1 µM rolipram

Experimental condition

Treatment during 10 min runcaciguat incubation

Treatment during cLTP protocol

Vehicle

0.1% DMSO

0.3% DMSO

Runcaciguat only (without WS)

10 nM runcaciguat

0.3% DMSO

Runcaciguat + WS

10 nM runcaciguat

50 µM forskolin + 0.1 µM rolipram

Weak stimulation (WS) only

0.1% DMSO

50 µM forskolin + 0.1 µM rolipram

  1. Described are the experimental conditions and corresponding exposures during the BAY-747 and runcaciguat incubation period and the chemical LTP protocol. WS: weak stimulation induced by 50 µM forskolin and 0.1 µM rolipram.
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